The gcms analysis of the acetone extract revealed the presence of five major compounds. Gc ms analysis of cassia italica leaf methanol extract asian j pharm clin res. Phytochemical analysis, antioxidant, antistress, and. Raj ms, kameshwari mn, tharasaraswath kj, shubharani r 2017 qualitative and quantitative analysis of phytochemicals in two different species of urginea. Pdf phytochemical analysis of some medicinal plants. Gc ms analysis methanol extract of solanum torvum leaves was analyzed with the help of gc ms analyzer perkin. Phytochemical analysis by gcms analysis of the plant rhizomes revealed the presence of different fatty acids, heterocyclic compounds etc. Medicinal plants continue to provide valuable therapeutic agents, both in modern and traditional medicine 3.
Principle and application of gcms detector in phytochemical analysis gcms plays a key role in the analysis of unknown components of plant origin. Acalypha indica distributed in the southern part of india particularly in tamilnadu and it has potential medicinal properties and used as diuretic, anthelmintic and for respiratory problems such as bronchitis, asthma and pneumonia. Gcms analysis of phytochemical compounds in aqueous. Phytochemical analysis in different extracts methanol, hexane revealed the presence of secondary metabolites like. Introduction medicinal plants have been used for countries as remedies for human disease 1. Gcms analysis was performed to identify some of the potent. Alangium salviifolium, phytochemical, antibacterial, gcms introduction in this present era, many natural product researchers has focused on the plant research in all over the world and a wealth of evidence shows immense potential of ethnomedicinal plants.
Phytochemical screening and gcms analysis of methanolic. Rjpt gcms analysis of phytochemical compounds present in. C nielsen from lampung, indonesia irawan candra 1 foliatini dr. Keywords medicinal plants metabolomics gcms lcms flavonoids genistein. Phytochemical screening and gcms analysis of methanolic leaf. Gcms analysis of the plant extracts was performed by using gcms equipped with a. For the widespread acceptance of herbal medicines, standardization, quality control of the herbal materials, as well as. Phytochemical screening and gcms analysis of acetone. Sagittaria trifolia is a medicinal foodstuff of china and east asia belonging to the. Phytochemical analysis of methanolic extracts of leaves of. Gcms analysis of phytocostituents of some wild zingiberaceae. The phytochemical screening and gcms analysis of the extracts are in good agreement with the presence of alkaloids. Gas chromatographymass spectrometry gcms analysis of the whole plant.
To conduct a gcms analysis on ethanolic leaves extracts 25 com. The name of the compound, retention time and the percentage of their presence indicated as peak area are listed in tables 14, respectively, for the four plants extracts. Phytochemical analysis and assessment of antioxidant and antimicrobial activities of some medicinal plant species from egyptian flora. Journal of medicinal plants studies phytochemical and find the in vitro antidiabetic activity of. Gcms analysis of phytocomponents in the ethanol extract. Pdf phytochemical studies and gas chromatographymass. Phytochemical, gcms analysis and antibacterial activity of a medicinal plant acalypha indica. In vitro antidiabetic potential and gcms analysis digera. Gcms analysis was performed on the methanolic extract of s. Phytochemical screening and gcms studies of the medicinal plant. Phytochemical screening and gcms, ftir analysis of.
Phytochemical analysis, gcms analysis, macrotyloma uniflorum, and oleic acid. The phytochemical profile of methanolic leaf extract from cecropia species was further explored using an hplc method coupled to both dad and ms detectors. Phytochemical, gcms analysis and antibacterial activity. Calotropis gigantea, phytochemical screening, gcms analysis. Natural phytochemicals contained in plants, such as alkaloids, flavonoids.
The gc ms chromatogram of these medicinal plant extracts was shown in the figure 3. Jabin f, nasreen s 2016 phytochemical analysis of some medicinal plants. Phytochemical analysis of traditional medicinal plants and. The crude extract form was subjected to column chromatography over silica gel 100200 mesh and eluted with nhexane, chloroform, ethanol. Cyperaceae s srinivasan and v priya abstract in the present study the investigation on phytochemical screening was carried along with gc ms analysis to identify the phytoconstituents present in extracts of cyperus dubius, rottb. Alangium salviifolium, phytochemical, antibacterial, gcms introduction in this present era, many natural product researchers has focused on the plant research in all over the world and a wealth of evidence shows immense potential of ethnomedicinal plants in various traditional system archana et al. Gcms analysis of bioactive compounds in methanolic extract of.
Gcms analysis of the essential oil and methanol extract. In this modern era, medicinal plants are at great attention to the researchers as most of the drug. At the same time, the phytochemical constituents like phlobatannin were absent table 1. Gcms analysis of phytochemical compounds in aqueous leaf.
It includes auto sampler and a gas chromatograph which interfaced to a mass spectrophotometer. Phytochemical screening and gcms studies of the medicinal. The phytochemical investigation was carried out to determine the possible bioactive components from the methanol extract of eclipta prostrata leaves by gas chromatography and mass spectroscopy gc ms. An experience from north east india j pharm res phytochemical analysis of traditional medicinal plants and their antimicrobial activity. Introduction the indian subcontinent is rich in medicinal plants and is one of the richest countries in terms of genetic diversity of medicinal plants.
Preliminary phytochemical screening and gc ms issn profiling. Department of chemistry, institute of science, civil lines, nagpur 440001, maharashtra, india. Oct 21, 2014 phytochemical analysis, antioxidant, antistress, and nootropic activities of aqueous and methanolic seed extracts of ladies finger abelmoschus esculentus l. Phytochemical screening and gas chromatographymass. Phytochemical analysis was carried out for identification of tannins, terpenoids, flavonoid, alkaloid, phenol, phytosterol and saponins according to standard methods trease and evans, 2008. Then filtrate was centrifuged at 2500 rpm for 15 minutes and filtrate was stored in sterile bottles at 5 0c for further use harbone, 1973. Phytochemical screening and gcms analysis of leaf extract. Phytochemical and gcms analysis of canthium coromandelicum. Medicinal plants are the most potent antimicrobial natural source. India is the largest producer of medicinal herbs and is appropriately called the. Gcms analysis and phytochemical screening of a rare. Gcms analysis of phytochemical compounds of normal and.
Phytochemical analysis of the methanolic extracts of nine egyptian plants confirmed that these extracts were rich in phenolics, flavonoids, and alkaloids. Principle and application of gc ms detector in phytochemical analysis gc ms plays a key role in the analysis of unknown components of plant origin. Plants have a great potential for producing new drugs of great benefit to mankind. Phytochemical screening and gcms studies of the medicinal plant pavetta indica. Phytochemical analysis of leaf extract of eclipta alba l. Eclipta prostrata is a perennial herb, distributed in the tropical and sub tropical regions of the world. Gcms analysis of phytocomponents in the ethanol extract of. Medicinal plant, phytochemical screening, uvvisible. Gc ms chromatogram of methanolic extracts of cuscuta reflexa muzaffarnagar sample. Graphical representation of data was performed by using sigma plot 10.
The gcms analysis revealed the presence of 16 major compounds, with. Phytochemical analysis of species of the genus cecropia the polyphenolic content of four authentic plant species and commercial products of the genus cecropia species 15 samples were analized. Lib libraries provided by the software of the gcms system turbomass. Phytochemical analysis phytochemical screening of crude extracts of pergularia daemia was carried out according to the methods described by trease and evans 15,16. Gcms analysis of the essential oil and methanol extract of. Phytochemical analysis and assessment of antioxidant and. Phytochemical analysis of leaf extract of ocimum americanum l. Phytochemical screening, gcms, ftir analysis of methanolic. An endangered medicinal plant mohan b, nayak jb, r sunil kumar, lp shiva kumari, mohan ch and rajani b abstract the present paper reports the phytochemical and gc ms analysis studies of an endemic endangered, climbing shrub. Gcms analysis methanol extract of solanum torvum leaves was analyzed with the help of gcms analyzer perkin. Phytochemical characterization and comparative studies of. Phytochemical, gcms analysis and antibacterial activity of a. Raj ms, kameshwari mn, tharasaraswath kj, shubharani r 2017 qualitative and. Gcms analysis of phytochemical compounds of normal and leaf.
Comparative phytochemical analysis of cuscuta reflexa roxb. Phytochemical screening and analysis of selected medicinal. Volatile compound analysis using gcms, phytochemical. In recent years gcms studies have been increasingly applied for the analysis of medicinal plants as this technique has proved to be a reliable method for the analysis of nonpolar components. Gcms analysis of phytochemical compounds pertanika j. Phytochemical screening, gcms analysis of decalepis. Antioxidant activity was analyzed by using 2,2diphenyl1picrylhydrazylhydrate assay.
Hence, the present study is carried out using gcms analysis for phytochemical components from ethanolic extract of pseudoglochidion anamalayanum gamble. Lamiaceae by gcms method shubhangi nagorao ingole department of botany, bar. Therefore, search for newer drugs with minimum side effects obtained from. Phytochemical analysis of traditional medicinal plants and their antimicrobial activity. Elite db 5m column and the gcms solution version 2. Hence, the present study is carried out using gcms analysis for phytochemical components from ethanolic extract of. Rjpt gcms analysis of phytochemical compounds present. Phytochemical and gcms analysis of bioactive compounds of. In recent years gcms studies have been increasingly applied for the analysis of medicinal plants as this technique has proved to be a reliable method for the analysis of nonpolar components and volatile essential oil, fatty acids, lipids and alkaloids 89. Phytochemical analysis and gcms analysis of leaves of. Preliminary phytochemical screening and gc ms issn. Gc ms ionizes compounds and measures their mass numbers.
The peaks of the compounds with retention time in mins identified by gcms analysis of methanolic extracts of four plants respectively figure. The name of the compound, retention time and the percentage of their presence indicated as peak area are listed in tables 14, respectively, for the four plants. Phytochemical analysis, molecular docking and molecular. The phytochemical screening showed the presence of alkaloids, tannins, steroids, flavonoids, saponins, anthraquinones, cardiacglycosides, phlobatinnins and carbohydrates. Preliminary phytochemical screening of the extract was carried out according to the standard method described by brindha et al. Preliminary phytochemical screening, quantitative analysis. An experience from north east india j pharm res phytochemical analysis of traditional medicinal plants. The gcms analysis of the essential oil of the seeds identified the presence of. Antimicrobial and phytochemical analysis of leaf extract of medicinal fruit plants. Phytochemical analysis of crude powder of the samples for the evaluation of phytochemicals. Nearly 12 compounds were indentified in the pantnagar sample. Through the chemical tests in the methanolic extracts of leaves of the five investigated plants. Gc ms analysis of phytochemical compounds pertanika j.
Phytochemical composition of potentilla anserina l. Phytochemical screening phytochemical analysis was carried out for identification of terpenoids, flavonoid, tannins, phenols, phytosterols, alkaloids and saponins according to standard methods kumar et al. The column was fused silica capillary, hp5 column 30. Phytochemical screening and gcms analysis of mukia. The compounds present in the hydroalcoholic extracts of mukia maderaspatana were identified by. Preliminary phytochemical screening and gcms analysis of methanol extract of ceropegia pusilla 51 water bath and it was filtered through whatman no.
Phytochemical screening and gcms analysis of hexanolic extract of lantana camara linn mamta uparkar and sunil h. Research article gcms analysis of phytochemical compounds present in the rhizomes of nervilia aragoana gaud elizabeth thomas1, aneesh t. Preliminary phytochemical screening, quantitative analysis of. Phytochemical screening and gcms analysis of cyperus.
The crude extract form was subjected to column chromatography over silica gel 100200 mesh and eluted with nhexane, chloroform, ethanol and methanol respectively. The methanol fractions of each plant were taken for gc ms analysis. Gcms analysis of this extract was performed using a perkin elmer gc claurus 500 system and gas chromatograph interfaced to a mass spectrometer gcms equipped with a elite1 fused silica capillary column 30 m. A study of phytochemical constituents in caralluma umbellata. To investigate the importance of any medicinal plant, the initial or first. It exhibits a wide range in topography and climate. Gc ms total ion chromatogram of the methanol extract of the leaves of kayea assamica table 2. Pharmacognosy gcms phytochemical analysis day is international event on 11 october, every year and commonly known as pharmacognosy gcms phytochemical analysis fair which provide leading platform to business activities in pharmacognosy gcms. Pharmacognosy gcms phytochemical analysis day is international event on 11 october, every year and commonly known as pharmacognosy gcms phytochemical analysis fair which provide leading platform to business activities in pharmacognosy gcms phytochemical analysis, sales and purchase of instruments, and services. Phytochemical analysis of a folklore medicinal plant citrullus colocynthis l bitter apple.
Preliminary phytochemical screening and gc ms analysis of methanol extract of ceropegia pusilla 51 water bath and it was filtered through whatman no. Phytochemical analysis by gcms analysis of the plant rhizomes revealed the presence of different fatty acids, heterocyclic. Phytochemical screening and gc ms analysis of methanolic extract of an endemic doi. Phytochemical analysis was carried out for identification of tannins, terpenoids, flavonoid, alkaloid, phenol, phytosterol and saponins according to standard methods trease and evans. The phytochemical analysis showed that the ephedra intermedia plant extract contains a mixture of phytochemicals as reducing sugars, cardiac glycoside, phenolic compounds, flavonoids, and alkaloids. Phytochemical screening of the entire herb of lactuca runcinata dc revealed the. Gcms analysis of bioactive phytochemicals present in ethanolic.
Multivariate statistics of the integrated gcms and lcms data showed. Gcms chromatogram of methanolic extracts of cuscuta reflexa muzaffarnagar sample. Phytochemical screening and gc ms analysis of cyperus dubius, rottb. Medicinal plants have been used for centuries as remedies for human disease. The aim of this study was to evaluate the antioxidant activity, screening the phytogenic chemical compounds, and to assess the alkaloids present in the e. A study of phytochemical constituents in caralluma. India is the largest producer of medicinal herbs and is appropriately called the botanical garden of the world1,2. Introduction plants are used as a source of medicine that has been inherited as one of the important component of the health care system. An experience from north east india minakshi b, jharna d, chanbi devi e, nayan t, partha pk, kundal n. Phytochemical, gcms analysis and antibacter ial activity of a medicinal plant acalypha indica s. The detailed tabulations of gcms analysis of the extracts are given in table 1, table 2, table 3, table 4 and table 5 respectively. The results also indicated potential antioxidants as well as antimicrobial activities against pdr s.
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